β-GLUCURONIDASE OF HUMAN WHITE BLOOD CELLS

Surface Glycoproteins of Human White Blood Cells

We labeled surface glycoproteins of norby polyacrylamide slab gel electrophoresis. mal human blood platelets, granulocytes, All major types of human leukocytes monocytes, T and B lymphocytes, and null showed different and characteristic surcells by the galactose oxidase-NaB3H4 face glycoprotein patterns. These patterns and periodate-NaB3H4 labeling techevidently also include common comniques. T...

Application of an improved glucuronidase assay method to the study of human blood beta-glucuronidase.

Previous conditions in which phenolphthalein glucuronide was employed as substrate (1) have proved unsatisfactory for the determination of glucuronidase in plasma, serum, and laked blood cells. Thus, low glucuronidase activities such as frequently occur in plasma could not be accurately measured or even approximated, owing to a combination of circumstances; i.e., too short an incubation period ...

Optimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation

Cryopreservation is the method of choice for long term storage of human PBMCs. This study was designed to compare the different combinations of variables affecting the cryopreservation of PBMCs samples. The viability of PBMCs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with ...

About White Blood Cells

Analysis of allelic differential expression in human white blood cells.

Allelic variation of gene expression is common in humans, and is of interest because of its potential contribution to variation in heritable traits. To identify human genes with allelic expression differences, we genotype DNA and examine mRNA isolated from the white blood cells of 12 unrelated individuals using oligonucleotide arrays containing 8406 exonic SNPs. Of the exonic SNPs, 1983, locate...